Work packages – Blueprint

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Blueprint > Work packages

Work packages of Blueprint

The Blueprint project contains seven interacting work packages (WPs):

Here you can find short descriptions of each WP content:

WP1 (Klaus Jürgens): Spatio-temporal variation of BLUEPRINTs (in situ data)

In this WP we aim to identify the linkage between different environmental conditions and the functional profile / diversity (the genetic blueprint) of microbes in the Baltic Sea. This will be done through extensive sampling of the fundamentally different subsystems of the Baltic, the abiotic gradients, and the seasonal variation.

WP2 (Veljo Kisand): Coupling of rates and genes

This WP aims at experimentally identifying key genetic and functional properties of natural microbial assemblages exposed to well-defined stressors which are known to constitute major environmental concerns in the Baltic Sea (e.g., hypoxia, N limitation leading to N2-fixing cyanobacterial blooms, and increased input of DOM/organic contaminants). The WP serves as an experimental examination of the links between environmental conditions and BLUEPRINTs identified in situ (WP1).

WP3 (Jarone Pinhassi): Identification of BLUEPRINTs in model bacteria

The objective of WP3 is to experimentally investigate responses in key diagnostic genes to selected external drivers/stressors. With this objective, we will carry out ecophysiological response experiments with genome-sequenced model bacteria and cyanobacteria to establish causal links between key environmental stressors and genetic signatures and selected nutrient fluxes/biogeochemical process rates of bacteria.

WP4 (Anders Andersson): Sample processing and statistical analysis (bioinformatics pipeline)

WP4 provides the bioinformatics infrastructure for extracting meaningful signals from the data collected in other WPs and for facilitating easy sharing of data across the WPs.

WP5 (Åke Hagström): Evaluation of genes and rates, design of reference experiments

Biogeochemical modeling is an essential tool to design management actions required to reach environmental targets set e.g. by HELCOM’s Baltic Sea Action Plan (BSAP) and the EU Marine Strategy Framework Directive (MSFD). It is therefore an essential task to generate additional scientific support for the formulation of Baltic biogeochemical models. The goals of WP5 are to a) use microbial blueprints to validate and improve parameterization of basin-scale biogeochemical models of the Baltic Sea. In particular, we will address key processes in biogeochemical cycling, e.g., carbon and nutrient mineralization, N2 fixation and denitrification, and b) provide an envelope covering a range of different environmental conditions, to optimize the sampling and experimental efforts in WP1 and WP2.

WP6 (Matthias Labrenz): BCC development and evaluation (standardization, communication)

The focus of WP6 is the transfer and integration of new microbial descriptors into existing monitoring procedures. Several aspects have to be considered, going deep into very different disciplines ranging from basic science over bioinformatics and potential development or adaptations of new instrumentations. WP6 will merge these aspects by improving, developing, evaluating, and standardizing general and specific working procedures to guide BLUEPRINT into practical operability within the virtual BLUEPRINT Competence Center (BCC).

WP7 (Lasse Riemann): Management and BCC dissemination

The objectives of this WP are to a) ensure efficient project management allowing for successful accomplishment of the described tasks and timely production of deliverables, and b) ensure dissemination of the project outcome to relevant institutions and policy makers.